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Focusing on the research and development and application of isothermal nucleic acid amplification technology

重组酶介导的核酸等温扩增荧...血吸虫感染性钉螺的效能评价_叶钰滢

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[Abstract] Objective: To evaluate the efficacy of recombinase-aided amplification (RAA) fluorescence assay in detecting infective Oncomelania snails infected with Schistosoma japonicum. Methods: A pooled-sample detection method was employed. Fifty snails were pooled into a single test sample, with negative samples containing no infective snails and positive samples harboring varying numbers of infective snails. Ten negative samples and 10 positive samples each containing 12 or 3 infective snails, respectively, were randomly divided into groups. These samples were then tested blindly using the fluorescent RAA method, with the miracidial hatching assay serving as the gold standard. Sensitivity, specificity, Youden’s index, and overall agreement rate of the fluorescent RAA assay were calculated. Additionally, five negative snail samples and five positive samples containing 1, 2, or 3 infective snails each were randomly assigned to two groups. Each sample was tested blindly twice—once by crushed-microscopic examination and once by fluorescent RAA—and the results were compared. Results: The fluorescent RAA assay correctly identified 29 out of 30 positive samples, yielding a sensitivity of 96.67%. Among 10 negative samples, 8 were correctly identified as negative, resulting in a specificity of 80.00%. The Youden’s index was 0.77, and repeated testing of the same samples 10 times achieved a perfect concordance rate of 100%. Furthermore, there was no statistically significant difference between the fluorescent RAA assay and the crushed-microscopic method in detecting infective snails (P > 0.05). The agreement rates between the two methods were 95.00% (19/20) and 90.00% (18/20), respectively, based on actual infection status. Conclusion: The fluorescent RAA assay demonstrates excellent performance in detecting infective Oncomelania snails infected with Schistosoma japonicum, suggesting its potential for application in screening programs targeting these snails in areas endemic for Japanese schistosomiasis.

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重组酶介导的等温核酸扩增技术检测多房棘球绦虫方法的建立及初步应用_周鸿让CDC

重组酶介导的核酸等温扩增荧光法快速检测日本血吸虫感染性钉螺-江苏血防所

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Previous page

重组酶介导的等温核酸扩增技术检测多房棘球绦虫方法的建立及初步应用_周鸿让CDC

Next page

重组酶介导的核酸等温扩增荧光法快速检测日本血吸虫感染性钉螺-江苏血防所