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Focusing on the research and development and application of isothermal nucleic acid amplification technology
Research Progress on the Application of Isothermal Amplification Technology Using Recombinase in Analytical Detection—Shanghai Ocean University
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Abstract: Recombinase polymerase amplification (RPA) and recombinase-aided amplification (RAA) are two highly representative and cutting-edge isothermal nucleic acid amplification technologies. Both RPA and RAA can perform isothermal amplification at constant temperatures and enable rapid amplification even under simple equipment conditions or in resource-limited settings. They offer advantages such as high specificity, high sensitivity, ease of operation, and short detection times. This article first briefly outlines the amplification principles underlying RPA and RAA technologies, then focuses on recent research advances in the application of recombinase-based isothermal amplification techniques to analytical detection. It also identifies existing challenges and proposes potential solutions, while offering a forward-looking perspective on their future development. The aim is to provide theoretical references for expanding the application scope of these two technologies in the future.
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