Innovation
Focusing on the research and development and application of isothermal nucleic acid amplification technology
重组酶等温扩增技术在分析检测中的应用研究进展-上海海洋大学
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Abstract: Recombinase polymerase amplification (RPA) and recombinase-aided amplification (RAA) are two highly representative and cutting-edge nucleic acid isothermal amplification technologies. Both RPA and RAA can perform isothermal amplification at room temperature, enabling rapid amplification even with simple equipment or in resource-limited settings. These techniques offer several advantages, including high specificity, exceptional sensitivity, ease of operation, and short detection times. In this review, we first briefly outline the amplification principles underlying RPA and RAA, then focus on recent advances in their application to analytical and diagnostic assays. We also highlight existing challenges and propose potential solutions, while展望ing the future prospects of these technologies. Our aim is to provide a theoretical reference for expanding the application scope of RPA and RAA in the coming years.
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