Innovative Technology

Innovation

Focusing on the research and development and application of isothermal nucleic acid amplification technology

动物源性食品中致病微生物的快速PCR检测-石家庄兽药中心

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Pathogenic microorganisms pose a significant threat to the safety and quality of animal-derived foods. Scientists and industry professionals have long been dedicated to researching and developing advanced detection technologies for these harmful microbes in animal-based products. Traditionally, detecting pathogenic microorganisms has relied primarily on biological culture and identification methods, which are time-consuming, demanding in terms of experimental conditions, and often require highly specialized technical expertise—limitations that have become increasingly apparent. However, with rapid advancements in science and technology, rapid PCR detection techniques have gained widespread adoption and continuous improvement due to their unique advantages: high specificity, exceptional sensitivity, and remarkable convenience. Starting from the original conventional PCR method, these techniques have evolved into more sophisticated approaches such as multiplex PCR and fluorescence PCR. In recent years, even innovative isothermal nucleic acid amplification technologies like LAMP, RAA, and RPA have emerged, quietly revolutionizing the field of rapid PCR-based detection of pathogenic microorganisms in animal-derived foods. This article provides a concise overview of six key rapid PCR detection technologies—conventional PCR, multiplex PCR, fluorescence PCR, LAMP, RAA, and RPA—and explores their applications in identifying pathogenic microorganisms. The aim is to offer valuable insights that can guide the promotion, further research, and ongoing refinement of detection methods for these harmful agents in animal-derived food products.