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Focusing on the research and development and application of isothermal nucleic acid amplification technology
Rapid PCR Detection of Pathogenic Microorganisms in Animal-Origin Foods – Shijiazhuang Veterinary Drug Center
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Pathogenic microorganisms pose a significant threat to the safety and quality of animal-derived foods. Scientists and industry professionals have long been dedicated to researching and developing detection technologies for pathogenic microorganisms in animal-derived foods. Traditionally, the detection of pathogenic microorganisms has relied primarily on biological culture and identification methods, which suffer from slow detection speeds and high requirements for experimental conditions and technical expertise—disadvantages that are particularly pronounced. However, with advances in technology, rapid PCR detection techniques have gained widespread adoption and continued development due to their advantages in specificity, sensitivity, and convenience. Starting from conventional PCR, these techniques have gradually evolved into multiplex PCR and fluorescent PCR. In recent years, even isothermal nucleic acid amplification technologies such as LAMP, RAA, and RPA have emerged, quietly bringing about a technological revolution in the field of rapid PCR detection of pathogenic microorganisms in animal-derived foods. This article briefly introduces six rapid PCR detection techniques—conventional PCR, multiplex PCR, fluorescent PCR, LAMP, RAA, and RPA—and their applications in the detection of pathogenic microorganisms, providing a reference for promoting, studying, and improving detection technologies for pathogenic microorganisms in animal-derived foods.
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