Innovative Technology

Innovation

Focusing on the research and development and application of isothermal nucleic acid amplification technology

西尼罗病毒的逆转录重组酶介导扩增检测方法-浙江国旅&北京国旅

  • File size: 1.3MB

Abstract: [Background] With West Nile fever spreading widely across the globe, the risk of its introduction into China has significantly increased. To address this growing threat, it is crucial to develop rapid detection methods for West Nile virus, thereby establishing a reliable methodological foundation for virus detection. [Objective] This study aimed to establish a reverse transcription recombinase-aided amplification (RT-RAA) assay for West Nile virus detection. [Methods] Primers and probes were designed based on conserved regions of the West Nile virus genome, enabling the development of an RT-RAA assay. The method’s repeatability, specificity, and sensitivity were subsequently evaluated. [Results] The RT-RAA assay features a constant reaction temperature of 39°C throughout the entire amplification process, resulting in a short detection time—within 20 minutes—and a detection limit as low as 10 copies. Importantly, the assay showed no cross-reactivity with mosquito-borne viruses such as Chikungunya virus, Dengue virus, Japanese encephalitis virus, or Yellow fever virus, demonstrating excellent specificity. Furthermore, sample testing results aligned with expected outcomes. [Conclusion] The established RT-RAA assay for West Nile virus exhibits rapid, specific, and highly sensitive characteristics, making it suitable for swift on-site detection at border checkpoints as well as for epidemiological surveillance purposes.

Previous page

塔希纳病毒的重组酶介导扩增快速检测方法-CDC&云南寄生虫防治所

重组酶介导扩增方法快速检测黄热病毒-浙江国旅

Next page

Previous page

塔希纳病毒的重组酶介导扩增快速检测方法-CDC&云南寄生虫防治所

Next page

重组酶介导扩增方法快速检测黄热病毒-浙江国旅