Innovative Technology

Innovation

Focusing on the research and development and application of isothermal nucleic acid amplification technology

应用重组酶介导的恒温扩增法建立检测非结核分枝杆菌的方法研究-浙江检验检疫&杭州疾控

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Abstract: Objective To establish a simple and rapid nucleic acid detection method for non-tuberculous mycobacteria under constant temperature conditions, providing a reference for clinical diagnostics. Methods Specific primers targeting three types of non-tuberculous mycobacteria—Mycobacterium marinum, Mycobacterium abscessus, and Mycobacterium kansasii—were designed. A recombinase-mediated isothermal amplification (RPA) technique was then employed to develop an efficient and swift assay for detecting these pathogens. Results The RPA fluorescence assay yielded conclusive results within approximately 20 minutes at 39°C. Notably, the detection limit of this method reached as low as 10 copies per liter, with no cross-reactivity observed against Mycobacterium tuberculosis or other clinically common pathogens, ensuring 100% specificity. Furthermore, the assay demonstrated excellent performance in detecting all relevant clinical isolates of non-tuberculous mycobacteria. Conclusion This method offers a highly specific, sensitive, and rapid diagnostic tool for non-tuberculous mycobacteria, suitable for use in straightforward laboratory settings.

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探针导向重组酶介导等温扩增法检测TB_rpoB基因突变的应用价值

（乙肝）-华北理工.pdf

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探针导向重组酶介导等温扩增法检测TB_rpoB基因突变的应用价值

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（乙肝）-华北理工.pdf