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Focusing on the research and development and application of isothermal nucleic acid amplification technology

重组酶介导恒温扩增快速检测人鼻病毒的方法学研究_陈淑丹

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Abstract: Objective To establish a rapid method for detecting human rhinovirus using reverse transcriptase recombinase-aided amplification (RT-RAA) technology. Methods Primers and probes were designed based on the conserved sequences of human rhinovirus in the NCBI gene database. Human rhinovirus RNA was first reverse-transcribed into cDNA, which was then used as the template for amplifying and detecting specific viral nucleic acid sequences via RT-RAA. The sensitivity of the assay was evaluated by analyzing plasmids containing varying concentrations of human rhinovirus DNA, while its specificity was confirmed by testing against known samples. Results The designed human rhinovirus-specific primers and probes effectively amplified the target viral nucleic acid without cross-reacting with other viruses. The reaction was performed at a constant temperature of 39°C, yielding amplification results within 30 minutes, with a detection limit as low as 100 copies. Conclusion The established RT-RAA method demonstrates high sensitivity and specificity for human rhinovirus detection, offering rapid results and simple operation, making it well-suited for the quick identification of this virus in clinical settings.

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中东呼吸综合征冠状病毒重组酶介导核酸检测方法的建立-宁波国旅&宁波检验检疫

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中东呼吸综合征冠状病毒重组酶介导核酸检测方法的建立-宁波国旅&宁波检验检疫

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