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Focusing on the research and development and application of isothermal nucleic acid amplification technology
Methodological Study on Rapid Detection of Human Rhinovirus by Isothermal Amplification Mediated by Recombinase—Chen Shudan
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Abstract: Objective: To establish a rapid method for detecting human rhinovirus using reverse transcriptase recombinase-aided amplification (RT-RAA). Methods: Primers and probes were designed based on the conserved sequences of human rhinovirus in the NCBI gene database. Human rhinovirus RNA was reverse-transcribed into cDNA, which was then used as a template for amplifying and detecting the viral nucleic acid sequence via RT-RAA. The sensitivity of this method was evaluated by analyzing plasmids containing different concentrations of human rhinovirus nucleic acid sequences, while its specificity was assessed using known samples. Results: The human rhinovirus-specific primers and probes designed in this study effectively amplified the corresponding viral nucleic acid without cross-reacting with other viruses. The reaction was performed at a constant temperature of 39°C, with an amplification time of 30 minutes and a detection limit of 100 copies. Conclusion: The established RT-RAA method for detecting human rhinovirus exhibits high sensitivity and specificity, features rapid reaction times and simple operation, making it suitable for the rapid detection of human rhinovirus.
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