Innovative Technology

Innovation

Focusing on the research and development and application of isothermal nucleic acid amplification technology

非洲猪瘟病毒实时荧光RAA检测方法的建立-南京农大&动卫

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To establish a simple and efficient grassroots clinical detection method for African Swine Fever Virus (ASFV), this study selected the conserved gene sequence B646L (P72) of ASFV, designed specific primers and probes, and developed a real-time fluorescent recombinase-aided amplification (RAA) assay for ASFV detection. The results showed that the newly established method exhibited comparable sensitivity to the OIE-recommended fluorescence quantitative PCR method, with a detection limit as low as 10 copies/μL, and could complete the test in just 10 minutes. Importantly, no cross-reactivity was observed with Foot-and-Mouth Disease Virus (FMDV), Porcine Parvovirus (PPV), Pseudorabies Virus (PRV), Porcine Circovirus Type 2 (PCV2), Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), or Classical Swine Fever Virus (CSFV). Furthermore, when applied to 100 clinical samples, the RAA assay yielded consistent results with those obtained by the OIE-recommended fluorescence quantitative PCR method—but crucially, the RAA method significantly reduced the overall testing time from approximately 2 hours to just 10 minutes. These findings demonstrate that the method developed in this study offers high sensitivity and excellent specificity, providing valuable technical support for the early clinical diagnosis of ASFV.

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Clinical Validation of Two Recombinase-Based Isothermal Amplification Assays (RPARAA) for the Rapid Detection of African Swine Fever Virus

牛病毒性腹泻病毒1型RT-RAA快速诊断方法的建立_范颖

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Clinical Validation of Two Recombinase-Based Isothermal Amplification Assays (RPARAA) for the Rapid Detection of African Swine Fever Virus

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牛病毒性腹泻病毒1型RT-RAA快速诊断方法的建立_范颖