Innovative Technology

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Focusing on the research and development and application of isothermal nucleic acid amplification technology

基于重组酶介导等温扩增技术的广州管圆线虫核酸检测方法的建立-江苏血防所

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A nucleic acid detection method for Angiostrongylus cantonensis was established, based on recombinase-aided isothermal amplification (RAA) technology. Methods: The internal transcribed spacer 1 (ITS1) gene sequence of Angiostrongylus cantonensis was selected as the target sequence. Specific primers and probes with the highest specificity were designed, synthesized, and screened. A robust RAA-based assay—both conventional and fluorescence-based—was then developed for the sensitive detection of Angiostrongylus cantonensis. Fluorescence RAA amplification was performed using templates containing varying copy numbers of the target gene fragment in recombinant plasmids, as well as genomic DNA from Angiostrongylus cantonensis at different concentrations, to evaluate the method's detection sensitivity. Additionally, fluorescence RAA assays were conducted using genomic DNA extracted from Angiostrongylus cantonensis, Schistosoma mansoni, Ascaris lumbricoides, Clonorchis sinensis, Echinococcus granulosus, Ancylostoma duodenale, and the snail hosts Biomphalaria glabrata and Parafossarulus manchuriensis, to assess the assay's specificity.

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基于荧光重组酶介导等温扩增...什曼原虫核酸检测方法的建立_林红

基于重组酶介导等温扩增技术的细粒棘球绦虫核酸检测方法的建立-江苏血防所

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基于荧光重组酶介导等温扩增...什曼原虫核酸检测方法的建立_林红

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基于重组酶介导等温扩增技术的细粒棘球绦虫核酸检测方法的建立-江苏血防所