Innovation
Focusing on the research and development and application of isothermal nucleic acid amplification technology
基于重组酶介导等温扩增技术的细粒棘球绦虫核酸检测方法的建立-江苏血防所
-
File size: 1.2MB
A nucleic acid detection method for Echinococcus granulosus was established, based on recombinase-aided isothermal amplification (RAA) technology. Methods: Specific RAA amplification primers and a fluorescent detection probe targeting a 12S rRNA gene sequence fragment of E. granulosus were designed, screened, and synthesized to develop a fluorescent RAA assay for this parasite. The assay was then evaluated for its detection sensitivity using recombinant plasmids containing varying copy numbers of the target sequence, as well as genomic DNA from E. granulosus at different concentrations. Additionally, the assay’s specificity was assessed by performing fluorescent RAA amplification reactions with genomic DNA samples derived from E. granulosus, Multiceps multilocularis, Schistosoma japonicum, Schistosoma mansoni, Ancylostoma duodenale, Clonorchis sinensis, Taenia saginata, Diphyllobothrium latum, and Taenia solium.
Previous page
Next page
Previous page
Next page
Product Inquiry
Contact: qt@qt-bio.com
Product Consultation:+86-510-85385531
Technical support:+86-18921157475
Address: No. 97,Xingye Building B,Linghu Avenue,Xiwu District, Wuxi City
WeChat Official Account
Official Official Account
www.300.cn