Innovative Technology

Innovation

Focusing on the research and development and application of isothermal nucleic acid amplification technology

Reverse Transcriptase Recombinase-Mediated Amplification Technology for Rapid Detection of Chikungunya Virus – Zhejiang National Travel

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In this study, we first reverse-transcribed the RNA of the Chikungunya virus into cDNA using reverse transcriptase. We then designed primers and probes targeting conserved sequences of the Chikungunya virus and developed a one-step isothermal nucleic acid amplification assay mediated by recombinant enzyme—RT-RAA (Reverse Transcription).
We developed a rapid detection method based on recombinase polymerase amplification (RPA) combined with reverse transcription–loop-mediated isothermal amplification (RT-LAMP), and evaluated its sensitivity and specificity. The results showed that the entire procedure of this method is carried out at 39°C, with a short detection time (approximately 20 minutes). The detection limit can reach as low as 100 copies, and the assay exhibits no cross-reactivity with mosquito-borne viruses such as yellow fever virus, Japanese encephalitis virus, West Nile virus, and dengue virus type 1. The RT-RPA assay for chikungunya virus established in this study demonstrates high sensitivity, strong specificity, and simple operation, making it well-suited for rapid detection of chikungunya virus at grassroots ports of entry.

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(Coxsackievirus) - CDC

Development of a reverse transcription recombinase-aided amplification assay for the detection of coxsackievirus A10 and coxsackievirus A6 RNA (Coxsackievirus)

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(Coxsackievirus) - CDC

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Development of a reverse transcription recombinase-aided amplification assay for the detection of coxsackievirus A10 and coxsackievirus A6 RNA (Coxsackievirus)