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Focusing on the research and development and application of isothermal nucleic acid amplification technology
逆转录重组酶介导扩增技术快速检测基孔肯雅热病毒-浙江国旅
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This study first reverse-transcribed Chikungunya virus RNA into cDNA using reverse transcriptase. Conserved sequences of the Chikungunya virus were selected to design primers and probes, enabling the establishment of a one-step, recombinase-aided isothermal nucleic acid amplification assay—RT-RAA (Reverse Transcription
The re∞mbinase-mediated amplification (RT△AA) rapid detection method was established, and its sensitivity and specificity were analyzed. Results showed that the entire process can be carried out at 39℃, with a short detection time of just 20 minutes. The assay achieved a detection limit as low as 100 ∞py, and no cross-reactivity was observed with mosquito-borne viruses such as yellow fever virus, Japanese encephalitis virus, West Nile virus, and dengue virus type 1. This RT-RAA assay for chikungunya virus, developed in this study, demonstrates high sensitivity, strong specificity, and ease of operation, making it well-suited for the rapid detection of chikungunya virus at grassroots port-of-entry settings.
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