Innovation
Focusing on the research and development and application of isothermal nucleic acid amplification technology
（柯萨奇病毒）-CDC(1).pdf
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Hand, foot, and mouth disease (HFMD) is a significant public health concern, with coxsackievirus A6 (CVA6) and coxsackievirus A10 (CVA10) being two of the major causative pathogens, alongside enterovirus 71 (EV71) and coxsackievirus A16 (CVAl6). In this study, a simple and rapid reverse transcription recombinase-aided amplification assay (RT-RAA) was developed for the detection of CVA10 and CVA6. The analytical sensitivity for detecting CVA10 and CVA6 at a 95% probability, determined by probit regression analysis, was 35 copies per reaction and 38 copies per reaction, respectively, with 100% specificity. When compared to commercial RT-PCR assays, the RT-RAA assay demonstrated excellent agreement when tested on 455 fecal specimens, yielding kappa values of 0.920 (p < 0.001) for CVA10 and 0.952 (p < 0.001) for CVA6, respectively. Furthermore, four samples that tested positive for CVA10 and five that were positive for CVA6 using the RT-RAA assay but negative by RT-PCR were confirmed as true positives. These findings highlight that the proposed RT-RAA assays are highly valuable tools for detecting CVA10 and CVA6 and hold great potential for use in resource-limited settings.
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