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Use of a rapid recombinase-aided amplification assay for Mycoplasma pneumoniae detection（肺炎支原体）-袁静老师
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Background: Mycoplasma pneumoniae is one of the most common causative pathogens of community-acquiredpneumonia (CAP), accounting for as many as 30-5096 of CAP during peak years. An early and rapid diagnosticmethod is key for guiding clinicians in their choice of antibiotics.Methods: The recombinase-aided amplification (RAA) assay is a recently developed, rapid detection method thathas been used for the detection of several pathogens. The assays were performed in a one-step single tubereaction at 39° Celsius within 1 5-30 min. in this study, we established an RAA assay for M. pneumoniae using clinicalspecimens for validation and commercial real-time PCR as the reference method.Results: The analytical sensitivity of the RAA assay was 2.23 copies per reaction, and no cross-reactions with any olthe other 15 related respiratory bacterial pathogens were observed. Compared with the commercial reaktime PCRassay used when testing 311 respiratory specimens, the RAA assay obtained 10096 sensitivity and 10096 specificitywith a kappa walue of 1.
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