Innovation
Focusing on the research and development and application of isothermal nucleic acid amplification technology
A Study on the Rapid Detection of Respiratory Adenovirus Based on RAA Fluorescence Assay—Hangguo Travel & Hangzhou Center for Disease Control & Taizhou Customs
-
File size: 341.2KB
Abstract: Objective To develop a rapid method for detecting respiratory adenovirus using recombinase-mediated nucleic acid amplification (RAA) technology. Methods Primers and probes were designed based on the conserved sequence of the adenovirus hexon gene in the NCBI GenBank database, and an RAA detection method was established. A series of gradient dilutions of recombinant plasmids carrying adenovirus hexon gene fragments were prepared to evaluate the sensitivity of the RAA method. The established RAA method was then used to detect human metapneumovirus, human bocavirus, respiratory syncytial virus, human rhinovirus, influenza A virus, and influenza B virus, thereby validating the specificity of the method. Results Amplification results were obtained within 20 minutes using recombinant plasmids containing different numbers of hexon gene fragments; the lowest detectable copy number was 10 copies per reaction. No cross-reactivity was observed with human metapneumovirus, human bocavirus, respiratory syncytial virus, human rhinovirus, influenza A virus, or influenza B virus. Conclusion The established RAA method exhibits high sensitivity and specificity, offers rapid reaction times, and is simple to perform, making it suitable for the rapid detection of respiratory adenovirus.
Product Inquiry
Contact: qt@qt-bio.com
Product Consultation:+86-510-85385531
Technical support:+86-18921157475
Address: No. 97,Xingye Building B,Linghu Avenue,Xiwu District, Wuxi City
WeChat Official Account
Official Official Account
www.300.cn