Innovative Technology

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Focusing on the research and development and application of isothermal nucleic acid amplification technology

基于RAA荧光法快速检测呼吸道腺病毒的研究-杭国旅&杭州疾控&台州海关

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Abstract: Objective To establish a rapid method for detecting respiratory adenovirus using recombinase-mediated nucleic acid amplification technology (RAA). Methods Primers and probes were designed based on the conserved sequence of the respiratory adenovirus hexon gene from the NCBI gene database, enabling the development of an RAA-based detection assay. A series of gradient dilutions was prepared using a recombinant plasmid carrying the adenovirus hexon gene fragment to evaluate the sensitivity of the RAA method. The specificity of the method was further validated by testing it against human metapneumovirus, human bocavirus, respiratory syncytial virus, human rhinovirus, influenza A virus, and influenza B virus. Results Amplification results were obtained within 20 minutes using recombinant plasmids containing varying numbers of hexon gene copies, with the lowest detectable copy number as low as 10 copies. Importantly, no cross-reactivity was observed with human metapneumovirus, human bocavirus, respiratory syncytial virus, human rhinovirus, influenza A virus, or influenza B virus. Conclusion The developed RAA method demonstrates high sensitivity and specificity, offers rapid results, and is simple to perform, making it well-suited for the quick detection of respiratory adenovirus.

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（呼吸道合胞病毒）-河北医科大&CDC

甲型流感病毒的逆转录重组酶介导核酸扩增快速检测方法研究-浙江国旅&浙江检验检疫

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（呼吸道合胞病毒）-河北医科大&CDC

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甲型流感病毒的逆转录重组酶介导核酸扩增快速检测方法研究-浙江国旅&浙江检验检疫