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Focusing on the research and development and application of isothermal nucleic acid amplification technology

重组酶介导等温扩增技术检测哈维氏弧菌-上海海洋大学

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Vibrio harveyi is a bioluminescent vibrio bacterium that commonly accumulates on the surfaces or in the intestines of marine animals, particularly prevalent in shrimp larvae [1]. According to research by Su and Hang [2], in the early stages of Vibrio harveyi infection in shrimp, larvae exhibit reduced mobility, even though the bacteria have not yet accumulated significantly enough to produce visible fluorescence. Meanwhile, the LuxS gene—a key gene associated with quorum sensing in Vibrio harveyi—can be detected during this initial phase, allowing farmers to determine whether the bacterial population has surpassed the control threshold. This early detection enables timely adjustments to the aquaculture environment, effectively preventing the proliferation and spread of Vibrio harveyi. In the mid-stage of infection, when shrimp larvae are already濒临死亡 (on the verge of death), faint bioluminescence becomes visible in the dark. At this point, if environmental conditions become favorable for Vibrio harveyi to multiply rapidly, it could lead to massive mortality among farmed shrimp—with no effective remedial measures available at this critical stage [3]. By the late stage of infection, after the shrimp larvae have died, the fluorescence reaches its peak intensity. At this point, the only viable option is to thoroughly clean and disinfect the entire aquaculture environment, which inevitably results in significant financial losses for farmers. Currently, while research into the pathogenic mechanisms of Vibrio harveyi has advanced considerably, the development of rapid, early-stage detection methods for this bacterium remains a persistent challenge. Existing techniques for detecting Vibrio harveyi primarily include traditional culture-based isolation methods, molecular biology approaches, and immunological assays.

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重组酶介导等温核酸扩增方法快速检测土壤沙门氏菌_郭雨

重组酶介导扩增方法快速检测A族乙型溶血性链球菌-杭州海关

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重组酶介导等温核酸扩增方法快速检测土壤沙门氏菌_郭雨

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重组酶介导扩增方法快速检测A族乙型溶血性链球菌-杭州海关