Innovation
Focusing on the research and development and application of isothermal nucleic acid amplification technology
Recombinase-mediated isothermal amplification technology for the detection of Vibrio harveyi—Shanghai Ocean University
-
File size: 805.3KB
Vibrio harveyi is a luminescent vibrio that accumulates on the surface or in the gut of marine animals, and is particularly common in shrimp larvae [1]. According to research by Su and Hang [2], in the early stages of Vibrio harveyi infection in shrimp, the activity of shrimp larvae tends to decline. At this stage, the bacterial load in the shrimp remains low, and no fluorescence has yet been observed. However, since the LuxS gene is associated with quorum sensing in Vibrio harveyi, even when no fluorescence is detectable in the early stages of infection, the presence of Vibrio harveyi can be assessed by detecting the LuxS gene in the shrimp. This allows for timely monitoring of whether the bacterial population has exceeded the control threshold, enabling prompt adjustments to the aquaculture environment and thus effectively controlling the reproduction and spread of Vibrio harveyi. In the mid-stage of infection—when shrimp larvae are nearing death—weak fluorescence can be observed in the dark. Once the growth conditions become favorable for Vibrio harveyi proliferation, massive mortality among cultured shrimp may occur. At this stage, no remedial measures are available [3]. In the late stage of infection—after the shrimp larvae have died—the fluorescence becomes most intense. At this point, the only viable option is to thoroughly clean and disinfect the entire aquaculture environment, which would cause significant economic losses for farmers. Currently, research on the pathogenic mechanisms of Vibrio harveyi has gradually become more comprehensive; however, the development of rapid, early detection methods for this bacterium has remained stagnant. At present, the main methods for detecting Vibrio harveyi include traditional culture-based isolation, molecular biological techniques, and immunological assays.
Product Inquiry
Contact: qt@qt-bio.com
Product Consultation:+86-510-85385531
Technical support:+86-18921157475
Address: No. 97,Xingye Building B,Linghu Avenue,Xiwu District, Wuxi City
WeChat Official Account
Official Official Account
www.300.cn