Innovative Technology

Innovation

Focusing on the research and development and application of isothermal nucleic acid amplification technology

猪附红细胞体重组酶介导等温扩增技术RAA荧光检测方法的建立-中国动卫

  • File size: 350.6KB

To establish an accurate and rapid detection method for swine Eperythrozoonosis, specific primers and probes were designed based on the conserved sequence of the α-enolase gene in Eperythrozoon suis. After screening and optimizing reaction conditions, a recombinase-aided amplification (RAA) fluorescence assay was successfully developed. The results demonstrated that this newly established method can specifically detect Eperythrozoon suis within 20 minutes at a constant temperature of 39°C. Importantly, the assay showed no cross-reactivity with Mycoplasma hyopneumoniae, Streptococcus suis, Escherichia coli, Classical Swine Fever virus, Porcine Circovirus type 2, African Swine Fever virus, or healthy pig blood samples. Additionally, the method achieved a detection limit as low as 1 copy/μL. When applied to 15 clinical samples, the RAA fluorescence assay yielded a positive rate of 60%, which closely matched the results obtained by the conventional fluorescent quantitative PCR method. Overall, the RAA fluorescence assay developed in this study exhibits excellent sensitivity and specificity, making it a simple and practical tool for the rapid clinical diagnosis of swine Eperythrozoonosis.

Previous page

重组酶介导的油菜茎基溃疡病...荧光核酸扩增检测体系的建立_陈吴健

猪圆环病毒4型实时荧光RAA检测方法的建立_陈伟月

Next page

Previous page

重组酶介导的油菜茎基溃疡病...荧光核酸扩增检测体系的建立_陈吴健

Next page

猪圆环病毒4型实时荧光RAA检测方法的建立_陈伟月