Innovation
Focusing on the research and development and application of isothermal nucleic acid amplification technology
Recombinase-Mediated Establishment of a Fluorescent Nucleic Acid Amplification Detection System for Clubroot in Rapeseed—Chen Wu Jian
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This study develops a rapid method for detecting the pathogen causing stem base ulcer disease in rapeseed using recombinase-mediated nucleic acid amplification (RAA). Primers and probes were designed based on the conserved sequence of the pathogen’s specific gene, LMR1-D. The sensitivity and specificity of the established RAA method were evaluated. Under reaction conditions at 39°C and with a reaction time of less than 20 minutes, the detection limit of this method can reach as low as 10 copies/mL. Moreover, the method exhibits no cross-reactivity with closely related species such as the pathogen causing blackleg in rapeseed, demonstrating excellent specificity. The RAA method developed in this study features rapid reaction kinetics, high specificity, and enhanced sensitivity, making it well-suited for the rapid detection of the pathogen causing stem base ulcer disease in rapeseed.
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