Innovative Technology

Innovation

Focusing on the research and development and application of isothermal nucleic acid amplification technology

重组酶介导的油菜茎基溃疡病...荧光核酸扩增检测体系的建立_陈吴健

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This study establishes a rapid method for detecting the pathogen causing clubroot in rapeseed, utilizing recombinase-aided amplification (RAA), an enzyme-mediated nucleic acid amplification technique. Primers and probes were designed based on the conserved sequence of the pathogen's specific gene LMR1-D, enabling thorough analysis of the sensitivity and specificity of the developed RAA assay. The method achieves a detection limit as low as 10 copies/mL within a reaction time of less than 20 minutes at 39°C, with no cross-reactivity observed against closely related pathogens such as those causing blackleg in rapeseed, demonstrating excellent specificity. Overall, the RAA-based assay established in this research offers fast reaction kinetics, high specificity, and exceptional sensitivity, making it highly suitable for the rapid detection of the clubroot pathogen in rapeseed.

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牛病毒性腹泻病毒1型RT-RAA快速诊断方法的建立_范颖

猪附红细胞体重组酶介导等温扩增技术RAA荧光检测方法的建立-中国动卫

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牛病毒性腹泻病毒1型RT-RAA快速诊断方法的建立_范颖

Next page

猪附红细胞体重组酶介导等温扩增技术RAA荧光检测方法的建立-中国动卫