Innovation
Focusing on the research and development and application of isothermal nucleic acid amplification technology
Establishment of a Rapid Method for Detecting Salmonella Using Recombinase-Aided Amplification Technology—Zhejiang Inspection and Quarantine.pdf
-
File size: 163.5KB
Objective: To establish a rapid detection method for Salmonella using recombinase-mediated nucleic acid amplification (Recombinase-Aided Amplification, RAA). Methods: Primers and probes were designed based on the invA gene of Salmonella. The sensitivity of the RAA method was evaluated by constructing a plasmid containing the target gene fragment. The specificity of the method was verified by testing Escherichia coli and Shigella, and the method was further validated using positive Salmonella samples. Results: The established method operates at 39°C and can complete the detection within 20 minutes. Its detection limit is 10² copies/μl. No cross-reactivity was observed with Escherichia coli or Shigella, demonstrating excellent specificity. Conclusion: The developed RAA detection method exhibits characteristics of rapidity, high sensitivity, specificity, and ease of operation, making it suitable for the rapid detection of Salmonella.
Product Inquiry
Contact: qt@qt-bio.com
Product Consultation:+86-510-85385531
Technical support:+86-18921157475
Address: No. 97,Xingye Building B,Linghu Avenue,Xiwu District, Wuxi City
WeChat Official Account
Official Official Account
www.300.cn