Innovation
Focusing on the research and development and application of isothermal nucleic acid amplification technology
重组酶介导扩增技术快速检测沙门菌方法的建立-浙江检验检疫
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Objective: To establish a rapid detection method for Salmonella using recombinase-aided amplification (RAA), an enzyme-mediated nucleic acid amplification technique. Methods: Primers and probes were designed based on the invA gene of Salmonella. The sensitivity of the RAA method was evaluated by constructing a plasmid containing the target gene fragment. Specificity was verified by testing against Escherichia coli and Shigella, while positive Salmonella samples were used to confirm the method’s reliability. Results: The established method operates at 39°C and achieves detection within 20 minutes, with a detection limit as low as 10² copies/μl. Importantly, no cross-reactivity was observed with Escherichia coli or Shigella, demonstrating excellent specificity. Conclusion: The developed RAA assay exhibits rapid, sensitive, specific, and easy-to-perform characteristics, making it highly suitable for the quick detection of Salmonella.
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