Innovation
Focusing on the research and development and application of isothermal nucleic acid amplification technology
新型等温扩增技术推动寄生虫病现场快速检测能力提升-海军军医大
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[Abstract] With the rapid advancement of molecular biology, isothermal amplification techniques—boasting advantages such as high efficiency, speed, and simplicity—have begun to be applied in nucleic acid detection of parasitic disease pathogens. These techniques have emerged as a crucial tool for advancing on-site detection and control efforts against parasitic diseases. A novel isothermal nucleic acid amplification method, the recombinase-aided isothermal amplification assay (Hecombinase-aided isothermal amplification assay), enables rapid amplification of target gene fragments within 5 to 20 minutes under isothermal conditions (typically between 37°C and 42°C). This method offers several key benefits, including ease of operation, swift amplification capabilities, minimal reliance on sophisticated equipment, and real-time result monitoring. Successfully employed in detecting various parasites and other pathogens, this technique has demonstrated remarkable sensitivity and specificity, making it particularly well-suited for large-scale, on-site testing of samples in environments beyond traditional laboratories. As a result, it holds significant potential for rapid field detection and widespread application.
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