Innovation
Focusing on the research and development and application of isothermal nucleic acid amplification technology
A Novel Isothermal Amplification Technology Boosts Rapid On-site Detection Capabilities for Parasitic Diseases—Naval Medical University.pdf
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[Abstract] With the rapid advancement of molecular biology, isothermal amplification technologies—owing to their advantages of high efficiency, speed, and simplicity—have now begun to be applied in nucleic acid detection of parasitic disease pathogens and have become an important tool for promoting on-site detection and control of parasitic diseases. A novel isothermal nucleic acid amplification method—the recombinase-mediated isothermal amplification assay (Hecombinase-aided isothermal amplification assay)—can amplify target gene fragments under isothermal conditions (typically between 37 and 42°C) within 5 to 20 minutes. This method boasts advantages such as simple operation, rapid amplification, no need for sophisticated instrumentation, and real-time result monitoring. It has been successfully applied to the nucleic acid detection of various parasites and other pathogens, demonstrating high sensitivity and specificity. Moreover, this method is particularly well-suited for large-scale, on-site sample testing in environments outside the laboratory, thus possessing significant potential for rapid on-site detection and wider application.
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