Epidemic Research Faces Timeliness Challenges

Since 2025, Chikungunya virus (CHIKV) has shown exceptionally active spread in tropical regions. Although traditional RT-PCR offers high sensitivity, its >2-hour amplification cycle and heavy reliance on specialized equipment have created bottlenecks for research teams needing critical data during outbreaks. Field traceability studies are further constrained by cold-chain transport requirements and laboratory dependencies.

Limitations of Current Detection Technologies:

• Virus Isolation: Time-consuming, labor-intensive, and incompatible with rapid screening
• Serological Diagnosis: Low sensitivity for early-stage infections, leading to missed cases
• RT-PCR: Requires expensive thermal cyclers and advanced labs; lacks portability
• RT-LAMP: Needs multiple primers and suffers from poor specificity
• NASBA: Lengthy 2-hour reaction time

Revolutionary Isothermal Amplification Technology

RAA technology, short for Recombinase Aided Amplification,is a technology that utilizes recombinase, single-stranded binding protein and DNA polymerase to perform nucleic acid amplification under isothermal conditions (the optimal temperature is 39℃).The specific principle is: Recombinases, single-stranded binding proteins, and primers form polymers to scan double-stranded DNA, unwind double-stranded DNA at sequences homologous to the primers, and single-stranded binding proteins (SSBS) bind to the untwisted single strand to prevent single-stranded DNA refolding. In the presence of

energy and dNTP, DNA polymerase completes the strand extension and repeats the process. The amplification can be detected by the instrument within 5-15 minutes.

Compatible detection methods include:

→ Electrophoresis Method

→ Test Strip Method

→ Fluorescent Method

RAA Scientifi Research Platform Kit：

RAA Research Platform Kit Features:

Academic Impact Validation

Supported by 190+ international journal publications:

Certified Institutional Partners

✅ Sierra Leone-China Friendship Laboratory (Malaria Surveillance)

✅ CDC (Emerging Virus Screening Systems)

✅ Leipzig University, Germany